Our team aims to create an entirely new way of RNA detection. This method can serve as an alternative for the expensive rtPCR method in miRNA- or other small RNA-detection in research or medicine. It is based on the unique idea of using an RNA amplification loop. We used magnetic beads coated with single-stranded probe DNAs, on which the target RNA acts as a primer for second-strand synthesis. The resulting dsDNA is the starting point of a transcription process in the same medium. The newly synthesised RNAs start the process again and again, thus producing a high amount of dsDNA -what we detected by the increased SYBR Green fluorescence in the test tube. We calibrated a simple fluorimeter and proposed a self-designed adaptor for PCR tubes as inexpensive hardware for qualitative analysis.
Viktória Kovács
17 years
Regina Tóth
17 years
| Stand | 1 |
| Project | Biology-01 |
| Country | Hungary |
