In physiological processes proteins are involved within a complex network of interactions. Moreover, almost all clinical drugs exert their effects via the modulation of proteins. In order to fully understand the function of proteins and develop improved medications, proteins must be studied with imaging techniques. Ligand based labeling can be used, if a protein-specific ligand exists. The unstable ligand-protein bond can be stabilized by supplementing the ligand with an additional molecule which forms a covalent bond with the protein upon exposure to 365 nm UV light. The developed UV-Covbel device can illuminate samples in multiwell cell culture plate with adjustable power and time pattern of 365 nm UV light for a stable, covalent ligand based labeling for life science research.
Benedek László Barna