Projects    »    

Developing a novel, inexpensive method to detect small RNAs in biological samples

Developing a novel, inexpensive method to detect small RNAs in biological samples

Our team aims to create an entirely new way of RNA detection. This method can serve as an alternative for the expensive rtPCR method in miRNA- or other small RNA-detection in research or medicine. It is based on the unique idea of using an RNA amplification loop. We used magnetic beads coated with single-stranded probe DNAs, on which the target RNA acts as a primer for second-strand synthesis. The resulting dsDNA is the starting point of a transcription process in the same medium. The newly synthesised RNAs start the process again and again, thus producing a high amount of dsDNA -what we detected by the increased SYBR Green fluorescence in the test tube. We calibrated a simple fluorimeter and proposed a self-designed adaptor for PCR tubes as inexpensive hardware for qualitative analysis.

Viktória Kovács

17 years

Regina Tóth

17 years

Stand1
ProjectBiology-01
CountryHungary

Share on Social Media

Watch the Award Cermony

17 September from 13:00 – 14:30