Escherichia Coli is a pathogenic bacteria which secretes Shiga toxins which can cause neurological complications and acute liver failure. Its outer lipid layer may act as a barrier to permeation of antibiotics, making it increasingly resistant to treatment. Therefore, methods of inhibiting bacterial growth are crucial to processes like water treatment. Ultraviolet light, specifically UVA, inhibits E. coli growth by creating reactive oxygen species which react with protein membranes and DNA leading to cell death. This investigation looks at optimizing bacterial inhibition by varying duration of exposure and light intensity. The results of this investigation may be used as an alternate method of water treatment in rural areas.