The aim of this project is to assess a method for sex-selective animal breeding. Ram sperm were sorted into two fractions, based on their sex chromosome. This was achieved by activating the TLR7/8 receptors on X chromosome carrying sperm with the ligand resiquimod, which causes the cells to precipitate. Real-time PCR was used to determine the fraction purity after sorting and the results were an average of 64% purity for X chromosome sperm and a 77% for Y chromosomes. Before and after sorting, spermograms were obtained, which showed high cell motility, decreasing slightly after sorting. The results indicate that sperm can be successfully sex-sorted using a simple and reliable protocol.
Petar Stoilov Zhotev