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Sorting of sperm cells to obtain offspring with the desired sex in domestic sheep (Ovis aries))

Sorting of sperm cells to obtain offspring with the desired sex in domestic sheep (Ovis aries))

The aim of this project is to assess a method for sex-selective animal breeding. Ram sperm were sorted into two fractions, based on their sex chromosome. This was achieved by activating the TLR7/8 receptors on X chromosome carrying sperm with the ligand resiquimod, which causes the cells to precipitate. Real-time PCR was used to determine the fraction purity after sorting and the results were an average of 64% purity for X chromosome sperm and a 77% for Y chromosomes. Before and after sorting, spermograms were obtained, which showed high cell motility, decreasing slightly after sorting. The results indicate that sperm can be successfully sex-sorted using a simple and reliable protocol.

Petar Stoilov Zhotev

17 years

Stand3
ProjectBiology-03
CountryBulgaria

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