The occurrence and behavior of insects is significantly affected by the environment they live in. In this thesis, I dealt with the influence of structure of landscape on nesting preferences of Hymenoptera. For this comparison I had chosen to work with twig-nesting Hymenoptera, for which I have placed artificial nest opportunities into four biotopes – heath, edge of a heath, country lanes between fields and field. The studied location is located south of Znojmo near Podyjí national park. Particularly, I have focused on small carpenter bees of genus Ceratina. My results show that there is a big difference in the species distribution between the habitates of field and heath. The habitats of edge of a heath and country lanes make up a gradient between these two biotopes.